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- 2025年12月23日
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- 详细信息
- 询价记录
- 技术资料
- 保质期：
１年
- 英文名：
Human Methylated & Non-methylated DNA Set
- 库存：
大量
- 供应商：
简石生物
- 保存条件：
-20C
HIGHLIGHTS
- Purified, non-methylated and methylated human DNA for use as negative and positive controls in methylation detection applications. Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
- Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP).
- Each standard is provided with a primer set to amplify a fragment of DNA after bisulfite conversion
DESCRIPTION
The Human Methylated & Non-methylated DNA Set consists of two DNA controls (a CpG-methylated human DNA standard and a non-methylated human DNA standard), with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation family of products. This DNA set can be included as a positive and negative control to assess the efficiency of bisulfite conversion of DNA. The non-methylated human DNA is purified from the HCT116 DKO cell line, which contains genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-). The methylated DNA standard is purified HCT116 DKO DNA that has been enzymatically methylated at CpG sites.
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