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- ZYMO RESEARCH
- 美国
- D5014
- 2026年10月18日
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- 询价记录
- 文献和实验
- 技术资料
- 保质期：
１年
- 英文名：
Human Methylated & Non-methylated DNA Set
- 库存：
大量
- 供应商：
简石生物
- 保存条件：
-20C
HIGHLIGHTS
- Purified, non-methylated and methylated human DNA for use as negative and positive controls in methylation detection applications. Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
- Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP).
- Each standard is provided with a primer set to amplify a fragment of DNA after bisulfite conversion
DESCRIPTION
The Human Methylated & Non-methylated DNA Set consists of two DNA controls (a CpG-methylated human DNA standard and a non-methylated human DNA standard), with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation family of products. This DNA set can be included as a positive and negative control to assess the efficiency of bisulfite conversion of DNA. The non-methylated human DNA is purified from the HCT116 DKO cell line, which contains genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-). The methylated DNA standard is purified HCT116 DKO DNA that has been enzymatically methylated at CpG sites.
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文献和实验Methylated DNA Immunoprecipitation (MeDIP)
Introduction Methylation of cytosines can mediate epigenetic gene silencing and is the most prominent DNA modification in eukaryotes. MeDIP is an immunocapturing approach to enrich DNA that is methylated. The principle
Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation
to methylated DNA. Methyl-CpG Immunoprecipitation (MCIp) employs an immobilized recombinant human methyl-CpG binding domain protein 2, MBD2, which binds methylated CpGs in double-stranded DNA. Elution with increasing salt concentrations allows the fractionated
Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNA
I provide a protocol for DNA methylation profiling based on immunoprecipitation of methylated DNA using commercially available monoclonal antibodies that specifically recognize 5-methylcytosine. Quantification of the level of enrichment
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