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- 详细信息
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- 技术资料
- 保质期:
1年
- 英文名:
Universal Methylated DNA Standard
- 库存:
大量
- 供应商:
简石生物
- 保存条件:
-20度
HIGHLIGHTS
- Purified, methylated human DNA (D5011) and methylated mouse DNA (D5012) for use as controls to assess bisulfite conversion efficiency. Compatible with the EZ DNA Methylation family of products and other bisulfite conversion protocols.
- All cytosines within a CpG dinucleotide context have been enzymatically methylated by M. SssI methyltransferase.
- Each standard is provided with a primer set to amplify a fragment of DNA after bisulfite conversion.
DESCRIPTION
The Universal Methylated DNA Standards are designed to be used as positive controls to assess the efficiency of DNA bisulfite conversion in human and mouse models. The control DNA can be run in parallel with samples to monitor the bisulfite conversion reaction. The primer sets are designed to amplify a fragment of the supplied DNA from the respective species following bisulfite treatment.
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文献和实验Applications of the Universal DNA Microarray in Molecular Medicine
during clinical trials in order to first validate the biomarkers that contribute to both drug response and disease stratification. PCR/LDR/Universal DNA microarray is a promising technology to help drive the transition from the current paradigms of clinical
CE DNA analyzer. We focused mostly on the sample preparation step that is critical for the stability of generated fractions and reproducibility of a generated result. The application of universal primer for fluorescent labeling and omitting the PCR
DNA Barcoding Methods for Invertebrates
, the barcoding challenge in invertebrates is that of barcoding animals in general. While standard extraction, cleaning, PCR methods, and universal primers work for many taxa, taxon-specific challenges arise because of the shear genetic and biochemical diversity











