Adapter, Tube, Skirted, 5x50 m

He Tube Mating with MSCV recipient

. 3. Set up Dilutions:  Set up dilutions as shown below, in 5 ml LB+0.2% rhamnose. Use 50 ml Falcons for this as they have good aeration and you get much better growth. Place these dilutions at 30℃ shaking for 2 hours. pMSCV:  25 μl  5ml    50 μl  5ml

Sephadex G-50 spun column purification

. 1) Plug a 1ml syringe barrel with siliconized glass wool and fill to the top with a sterile suspension of Sephadex G50 equilibrated with H2 O. 2) Once filled place the syringe barrel into a 10ml centrifuge tube and spin at 3,000g

Sephadex G-50 spun column purification

syringe barrel with siliconized glass wool and fill to the top with a sterile suspension of Sephadex G50 equilibrated with H2 O. 2) Once filled place the syringe barrel into a 10ml centrifuge tube and spin at 3,000g for 5 minutes in a MSE Centaur