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- 详细信息
- 文献和实验
- 技术资料
- 规格:
50/unit, 10 units/case
- Ultra-pure polypropylene meets FDA 21 CFR 177.1520 & USP Class VI.
- High-contrast black 100 µL graduations are bold and easy to read.
- Large white writing area on all tubes.
- Optimized for vapor-phase liquid nitrogen storage.
Note: not suitable for immersion in liquid nitrogen. - Temperature range: -196°C to 121°C.
- Polypropylene cap has silicone rubber O-ring for a secure, leakproof seal.
- Maximize tube storage within racks, boxes, and canes.
- Sterile and certified free of detectable DNase, RNase, DNA, PCR inhibitors, and endotoxins.
-
SKU: 6122-S0
Volume: 1.8 mL
Cap Thread: Internally-Threaded
Seal: Silicone O-Ring
Base: Stargrip
Packaging: 50/unit, 10 units/case6122-S0, 6122-S0S
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文献和实验1. Medium preparation 1) Base Medium consists of 99mL of Williams’ Medium E combined with 1 mL of GlutaMAX™ I 2) Thaw the HepaRG™ Supplement by placing the bottle in a 37°C water bath until completely thawed. 3) Prepare the HepaRG
or DNA-buffer. (It is possible to store the pellet at -80℃; in that case,add 1 ml 1X PBS and resuspend the pellet.Use a cryo-tube and centrifuge at 1500 rpm for 2 min at 4℃.Remove the supernatant,and freeze the pellet.) 4.Remove supernatant
DNA Preparation from Fresh/Frozen Tissue
-buffer.(It is possible to store the pellet at -80°C;in that case,add 1 ml 1X PBS and resuspend the pellet.Use a cryo-tube and centrifuge at 1500 rpm for 2 min at 4°C.Remove the supernatant,and freeze the pellet.) 4.Remove supernatant and resuspend
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