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- Beckman Coulter贝克曼库尔特
- 美国
- C63369
- 2025年07月08日
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文献和实验He Tube Mating with MSCV recipient
. 3. Set up Dilutions: Set up dilutions as shown below, in 5 ml LB+0.2% rhamnose. Use 50 ml Falcons for this as they have good aeration and you get much better growth. Place these dilutions at 30℃ shaking for 2 hours. pMSCV: 25 μl 5ml 50 μl 5ml
The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
Lipoprotein Isolation - First week
100 ml beaker and add 25 µl of 200 mM PMSF Caution! Highly poisonous! picture ). Make sure no insect saliva drips into the beaker (have Kleenex ready to wipe off saliva). 3. Gently inject a few hundred microlitres of PBS
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