- ￥10879
- Beckman Coulter贝克曼库尔特
- 美国
- B89431
- 2025年07月11日
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文献和实验Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
into each microcentrifuge tube (~500 islets/tube/mouse). Allow islets to settle to the bottom of the microcentrifuge tubes. Draw one islet pellet into a P200 pipetman (set at 130ul) using a straight thin-wall pipet tip. Place a silicone tube adapter
Isolation of murine splenocytes
in 3mL DMEM-10, discarding any dead cell mass. Count cells (dilute 10μL cell suspension in trypan blue, and count with hemcytometer). Protocol B Set-Up same as Procedure B, except replace step 4 with: Sterilize
using the GS3 rotor. Repeat if necessary. 15b. Combine the DNA-containing supernatants and precipitate the DNA in 35 ml Corex glass tubes adding 2.5 volumes of cold 95% ethanol/acetate. 16b. Resuspend the dried DNA pellet in 2 ml of 10:0.1 TE
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