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文献和实验5 x SuperScript RTase buffer (Life Technologies) 100mM DTT 2.5mM dNTPs RNase inhibitor (Pharmacia) SuperScript RTase (Life Technologies) 10 x PCR buffer (Boehringer Mannheim, contains 15mM MgCl2 ) 500mM dNTPs Mineral oil RNA
this to 70℃ for 10 minutes, then place on ice for 5 minutes.3. Spin pulse the tube and then add the following reagents:4μl 5X SuperScript RTase buffer2μl 100mM DTT1μl 2.5mM dNTPs1μl RNase inhibitor1μl SuperScript RTase.4. Mix and incubate at 37℃ for 2 hours
实验试剂 Sterile water (high purity, RNase-free) 5 x SuperScript RTase buffer (Life Technologies) 100mM DTT 2.5mM dNTPs RNase inhibitor (Pharmacia) SuperScript RTase
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