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文献和实验He Tube Mating with MSCV recipient
cassette is between the H1H2 recombination regions which lie just inside two I-SceI cut sites (Sce1-H1-Cassette-H2-Sce1). Vector is Kanamycin (20), hairpin library insert is Chloramphenicol (50). Tube Mating Protocol Preparation: 1. Streak fresh donor
Cotton Transformation via Pollen Tube Pathway
transformation method—pollen tube pathway-mediated transformation. This method uses pollen tube pathway to deliver transgene into cotton embryo sacs and then insert foreign genes into cotton genome. There are three major steps for pollen tube pathway-mediated
Construction and Manipulation of Large-Insert Bacterial Clone Libraries
Introduction Construction of Large-Insert Bacterial Clone Libraries Fig. 1. A general procedure
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