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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD94
- 级别:
CD94 Antibody
- 目录编号:
CD94 Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
CD94 Antibody
- 抗体英文名:
Cyanine5.5 anti-mouse CD94 Antibody-PerCP
- 抗体名:
CD94 Antibody
- 标记物:
PerCP
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
CD94 Antibody
- 亚型:
CD94 Antibody
- 形态:
CD94 Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/ml
- 规格:
25 µg/100 µg
| 规格: | 25 µg | 产品价格: | ¥3300.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥7000.0 |
Description :CD94 is a 43/39 kD C-type lectin, also known as Kp43. It is present on all NK cells, NKT cells,and a subset of CD8-positive T lymphocytes in most mouse strains. CD94 is a type-IItransmembrane protein with an extracellular lectin-like domain and a short cytoplasmic tail.CD94 is expressed as a disulphide-linked heterodimer with a NKG2 subunit believed to mediatesignal transduction. When associated with NKG2A, the complex triggers inhibition; whenassociated with NKG2C, the complex triggers stimulation. The receptor complex of CD94 andNKG2 receptors bind to the ligand, Qa-1, and are thought to play a role in maintaining selftolerance in developing NK cells.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :CHO cells expressing the C57BL/6 alleles of CD94 and NKG2A
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5under optimal conditions.
Concentration :0.2 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser :Blue Laser (488 nm)
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文献和实验1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Tang X, et al. 2006. J. Immunol. 177:7645. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
大部分老师做流式实验时,会用到空白对照、单阳对照、同型对照、生物学对照等,用到 FMO 对照的比较少。尽管如此,FMO 对照在设置圈门时仍具有重要的参考价值。 一方面,FMO 对照有助于精确地设置阴阳性群体界限。我们在做多色实验时,可能会出现某些通道信号难以区分的情况,主要是因为其他某个荧光对该通道的干扰很大。FMO 对照(Fluorescence Minus One,荧光扣除一)减少了其中一个通道的荧光抗体,能够帮助衡量其它通道的荧光漏溢情况。 如下图所示,PerCP/Cyanine
条带。 五、 二抗的选择 二抗应选用与使用的一抗相同的物种来源,例如:如果你的一抗是小鼠的单克隆抗体,二抗则选抗小鼠的二抗anti-mouse secondary。建议检查二抗说明书确保该抗体适用于你的检测应用, 二抗一般连接荧光素FITC 或发光团。 六、双重染色抗体的选择 用未偶联一抗进行细胞培养物或组织切片的双重免疫染色要求一抗来源于不同物种并且二抗分别识别其中之一,二抗说明书应描述其与其它物种来源的免疫球蛋有否有交叉吸附。 七、荧光和化学发光标记 连接于二抗的标记










