- ￥1200 - 7200
- Proteintech
- HRP-66009
- 2025年10月13日
- WB
- Mouse
- human, mouse, rat, hamster, zebrafish, monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
HRP
- 适应物种：
human, mouse, rat, hamster, zebrafish, monkey
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB
- 靶点：
Beta Actin
- 抗体英文名：
HRP-conjugated Beta Actin Monoclonal antibody
- 抗体名：
HRP-conjugated Beta Actin Monoclonal antibody
- 规格：
100ul/500ul/1ml
| 规格： | 100ul | 产品价格： | ￥1200.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4800.0 |
| 规格： | 1ml | 产品价格： | ￥7200.0 |
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
HRP-66009 targets Beta Actin in WB applications and shows reactivity with human, mouse, rat, hamster, zebrafish, monkey samples.
| 经测试应用 | WB |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, hamster, zebrafish, monkey |
| 文献引用反应性 | human, mouse, rat, monkey |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | actin, beta |
| 别名 | ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, HRP-66009, PS1TP5BP1, β actin |
| 计算分子量 | 42 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | NM_001101 |
| 基因名称 | Beta Actin |
| Gene ID (NCBI) | 60 |
| RRID | AB_2883836 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P60709 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody is conjugated with HRP. It can recognize all the actins. The isotype of this antibody is IgG2b.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Protein Cell Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectodermAuthors - Kuisheng Liu |
| WB | Nat Commun Cytosine base editors induce off-target mutations and adverse phenotypic effects in transgenic miceAuthors - Nana Yan | |
| mouse | WB | Theranostics Platelets camouflaged nanovehicle improved bladder cancer immunotherapy by triggering pyroptosisAuthors - Jiale Tian |
| human | WB | Nat Commun Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.Authors - Hu Lei |
| human,mouse | WB | Nat Commun SOD1 regulates ribosome biogenesis in KRAS mutant non-small cell lung cancer.Authors - Xiaowen Wang |
| human | WB | Bone Res Restoring the dampened expression of the core clock molecule BMAL1 protects against compression-induced intervertebral disc degeneration.Authors - Dong Wang |
. For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP + 0.1% BSA
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
according to my protocol. For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP
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