Stat3 Antibody

9132:

ChIP Agarose , ChIP Magnetic , IHC / Paraffin , Immunoprecipitation , Western Blotting

Stat3 Antibody detects endogenous levels of total Stat3 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of mouse Stat3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or IFN-alpha treated (100 ng/ml for 5 min.), using Stat3 Antibody (left) or Phospho-Stat3 (Tyr705) Antibody #9131(right).

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using Stat3 Antibody.

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ Hep G2 cells starved overnight and treated with IL-6 (100 ng/ml) for 30 minutes, and either 10 μl of Stat3 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP^® Human c-Fos Promoter Primers #4663, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
7. Ihle, J.N. (1995) Nature 377, 591-4.
8. Wen, Z. et al. (1995) Cell 82, 241-50.
9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

• Bromberg, J. F. et al. (1999) Stat3 as an oncogene. Cell 98, 295-303. Applications: Western Blotting
• Craig, R. et al. (2000) p38 MAPK and NF-kappa B collaborate to induce interleukin-6 gene expression and release. J. Biol. Chem. 275, 23814-23824. Applications: IP Western Blotting
• Hutt, J. A. et al. (2000) Signal transducer and activator of transcription 3 activates CCAAT enhancer-binding protein delta gene transcription in G0 growth-arrested mouse mammary epithelial cells and in involuting mouse mammary gland. J. Biol. Chem. 275, 29123-29131. Applications: IP Western Blotting
• Peterson, W. M. et al. (2000) Ciliary neurotrophic factor and stress stimuli activate the Jak-STAT pathway in retinal neurons and glia. J. Neurosci. 20, 4081-4090. Applications: Western Blotting
• Ram, P. T. et al. (2000) Stat3-mediated transformation of NIH-3T3 cells by the constitutively active Q205L Galphao protein. Science 287, 142-144. Applications: Western Blotting
• Stairs, D.B. et al. (2011) Cancer Cell 19, 470-83. Applications: Western Blotting
• Mainardi, M. et al. (2010) Proc Natl Acad Sci U S A 107, 16673-8. Applications: IF-F

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 9134 Phospho-Stat3 (Ser727) Antibody
• 9914 Phospho-Stat Antibody Sampler Kit
• 9138 Phospho-Stat3 (Tyr705) (3E2) Mouse mAb
• 4904 Stat3 (79D7) Rabbit mAb
• 9139 Stat3 (124H6) Mouse mAb
• 9939 Stat Antibody Sampler Kit
• 9145 Phospho-Stat3 (Tyr705) (D3A7) XP^® Rabbit mAb
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8105 SignalSlide^® Phospho-Stat1/3/5 IHC Controls
• 8112 SignalStain^® Antibody Diluent
• 5245 STAMBP Antibody
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)

For Research Use Only. Not For Use In Diagnostic Procedures.