NF-κB p65 Antibody

3034:

Immunoprecipitation , Western Blotting

NF-κB p65 Antibody detects endogenous levels of total NF-κB p65. This antibody may also cross-react with the p50 subunit.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Ser276 of human NF-κB p65.

Western Blotting

Western blot anlaysis of extracts from HeLa cells, untreated or TNF-α-treated (#2169, 20 ng/ml) for the indicated times, using Phospho-NF-κB p65 (Ser536) Antibody #3031 (top) or NF-κB p65 Antibody (bottom).

Western Blotting

Western blot analysis of extracts from HeLa cells transfected with nontargeted (-) or targeted (+) siRNA. p65 was detect using NF-κB p65 Antibody #3034, and p42 was detected using p42 MAPK Antibody #9108. The NF-κB p65 Antibody confirms silencing of p65 expression, and the p42 MAPK Antibody is used to control for loading and siRNA specificity.

Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
3. Haskill, S. et al. (1991) Cell 65, 1281-9.
4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
9. Senftleben, U. et al. (2001) Science 293, 1495-9.
10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.

• Lee, J. et al. (2004) J Biol Chem 279, 53725-35. Applications: Western Blotting
• Abid, M.R. et al. (2004) J Biol Chem 279, 44030-8. Applications: Western Blotting
• Strassheim, D. et al. (2004) J Immunol 172, 5727-33. Applications: Western Blotting
• Minami, M. et al. (2008) J Biol Chem , . Applications: Western Blotting

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• 4764 NF-κB p65 (C22B4) Rabbit mAb
• 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
• 3039 Phospho-NF-κB p65 (Ser468) Antibody
• 4812 IκBα (44D4) Rabbit mAb
• 4814 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)
• 2859 Phospho-IκBα (Ser32) (14D4) Rabbit mAb
• 4954 RelB Antibody
• 4727 c-Rel Antibody
• 9936 NF-κB Pathway Sampler Kit
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.