- 询价
- Cell Signaling Technology
- 4725
- USA
- 2025年10月31日
- W
- H,M,R,Mk
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- 详细信息
- 技术资料
- 抗体英文名：
p48 Primase (8G10) Rat mAb
- 抗原：
full-length recombinant human p48 primase
- 应用范围：
W
- 保质期：
详见说明书
- 适应物种：
H,M,R,Mk
- 级别：
详见MSDS文件
- 供应商：
CST
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 48 | Rat IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | p48 Primase (8G10) Rat mAb detects endogenous levels of total p48 primase protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with full-length recombinant human p48 primase. Western Blotting
Western blot analysis of extracts from various cell types using p48 Primase (8G10) Rat mAb. |
| Background | Initiation of eukaryotic DNA replication is a stringently regulated process that requires the cooperation of many proteins and protein complexes to occur efficiently, at the origins of replication, and once per cell cycle. The initiation of DNA replication requires a protein complex composed of two DNA polymerase α subunits and a pair of primase subunits. Primase activity catalyzes de novo synthesis of an RNA/DNA primer (initiator DNA) on the leading and lagging strands, while polymerase activity extends the initiator DNA (1). The 48 and 58 kDa primase subunits cooperate in the synthesis of small RNA primers. p48 is the catalytically active subunit (2), while p58 couples p48 to the polymerase to allow the transfer of primers to the active site. The p58 subunit may also play a role in regulation of primer length (3,4). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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