- 询价
- Cell Signaling Technology
- 3017
- USA
- 2026年05月28日
- W, IHC-P, F
- H,Mk
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- 文献和实验
- 技术资料
- 抗体英文名：
NF-κB2 p100/p52 (18D10) Rabbit mAb (Human Specific)
- 抗原：
synthetic peptide corresponding to residues at the amino-terminus of human NF-kappaB2 p100/p52
- 应用范围：
W, IHC-P, F
- 库存：
大量
- 适应物种：
H,Mk
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) F=Flow Cytometry
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P F | H Mk | Endogenous | 52 active form. 120 precursor. | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | NF-kappaB2 p100/p52 (18D10) Rabbit mAb detects endogenous levels of both p100, the precursor, and p52 protein, the active form of NF-kappaB2. The antibody does not cross-react with other family members. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the amino-terminus of human NF-kappaB2 p100/p52. Western Blotting
Western blot analysis of extracts from HeLa, and COS cells, using NF-kB2 p100/p52 (18D10) Rabbit mAb (Human Specific). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using NF-kappaB2 p100/p52 (18D10) Rabbit mAb (Human Specific). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human osteosarcoma, using NF-kappaB2 p100/p52 (18D10) Rabbit mAb (Human Specific). Flow Cytometry
Flow cytometric analysis of HeLa cells, using NF-kappaB2 p100/p52 (18D10) Rabbit mAb (Human Specific) (blue) compared to a nonspecific negative control antibody (red). |
| Background | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
-1β. The family of TLRs and pro-inflammatory signal-transduction pathways that recruit NFκ B. The extraCell ular domains of Toll-like receptors (TLRs) include multiple leucine-rich repeats; TLR cytoplasmic domains are similar to the cytoplasmic portion
32000篇！CUSABIO 8月文献引用再创新高，Bioactive Materials、Nat Microbiol等顶刊齐发力
化应激和炎症，触发附睾上皮细胞焦亡（pyroptosis），进而损伤精子活力，而基质重塑相关蛋白7（MXRA7）通过抑制NF-κB信号通路发挥保护作用。临床EIF患者和EIF小鼠模型中，附睾氧化应激和炎症因子显著升高，精子活力明显下降，且附睾头部（caput）炎症重于尾部（cauda）。机制上，PKCα磷酸化介导MXRA7表达和磷酸化，MXRA7进而抑制NF-κB通路（尤其非经典p52/p100分支），减轻炎症和焦亡。附睾尾部高表达MXRA7是其抗炎能力强于头部的重要原因。首次将焦亡引入EIF生殖损伤
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