- 询价
- Cell Signaling Technology
- 2624
- USA
- 2026年01月11日
- western blot,免疫沉淀（IP）,免疫荧光（IF）
- 小鼠
- 不限
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°c
- 库存：
大量
- 抗体英文名：
GST (26H1) Mouse mAb
- 是否单克隆：
1
- 适应物种：
不限
- 应用范围：
western blot,免疫沉淀（IP）,免疫荧光（IF）
- 级别：
详见MSDS文件
- 供应商：
CST
- 抗原：
/
- 抗原来源：
/
- 保质期：
详见说明书
- 宿主：
小鼠
- 规格：
100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Complementary Reagents
GST (26H1) Mouse mAb #2624
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W IP IF-IC | All | Transfected Only | Mouse IgG2a |
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
Specificity / Sensitivity
GST (26H1) Mouse mAb detects transfected glutathione S-transferase (GST) fusion proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST fusion protein.
Western Blotting
Western blot analysis of extracts from transfected cells overexpressing GST-Bad and untransfected control, using GST (26H1) Mouse mAb (left) or anti-Bad Antibody (right).
IF-IC
Immunocytochemical staining of cells expressing GST-PAK2 protein (left) or cells without transfected protein (right), using GST (26H1) Mouse mAb.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
- Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
- Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
- Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
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For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验Mouse Models for miRNA Expression: The ROSA26 Locus
In 1991, Soriano and coworkers isolated the ROSA26 locus in a gene-trap mutagenesis screening performed in mouse embryonic stem (ES) cells. The ubiquitous expression of ROSA26 in embryonic and adult tissues, together with the high frequency
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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