- 询价
- Cell Signaling Technology
- 2208
- USA
- 2026年10月10日
- W, IP, IF-IC
- H,M,R,Hm,Mk
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- 文献和实验
- 技术资料
- 抗体英文名：
RPA32 (4E4) Rat mAb
- 抗原：
recombinant full-length human MBP-RPA32 protein
- 应用范围：
W, IP, IF-IC
- 适应物种：
H,M,R,Hm,Mk
- 库存：
大量
- 级别：
详见MSDS文件
- 供应商：
CST
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC | H M R Hm Mk | Endogenous | 32 | Rat IgG1 |
| Protocols | |
|---|---|
| Specificity / Sensitivity | RPA32 (4E4) Rat mAb detects endogenous levels of total RPA32 protein. The antibody recognizes the carboxy-terminal seqquence of RPA32. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with recombinant full-length human MBP-RPA32 protein. Western Blotting
Western blot analysis of extracts from various cell lines, using RPA32 (4E4) Rat mAb. IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (right), using RPA32 (4E4) Rat mAb (green) showing translocation to distinct nuclear foci after UV damage. Actin filaments have been labeled with Alexa Fluor® 555 phalloidin. Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
| Background | RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14 kDa subunits, collectively known as RPA. RPA is a single stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication (1-3), recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch and double-strand break repairs (4-7). In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 (9-1-1) checkpoint complex (8). RPA is hyperphosphorylated upon DNA damage or replication stress by checkpoint kinases including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) (9-11). Hyperphosphorylation may alter RPA-DNA and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA and Pol α, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen (10,12).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Treatment of Rat Glioma With Electrochemotherapy
of bleomycin followed by intracranial electrochemotherapy with exponential decaying pulses. In collaboration with the department of tumor immunology, Lund University, an ethyl-nitroso-urea induced rat glioma cell line (N32) is developed that produces glioma
Immunofluorescent Staining of Mouse and Rat Leukocytes
16 (Mouse BD Fc Block™; Cat No. 553141/553142) or anti-rat CD32 (Rat BD Fc Block™; Cat. No. 550270/550271) is recommended. BD Fc Block™ can be added to cells (~0.25 µg per million cells, 3 - 5 min, 4°C) and need not be washed out prior to addition of primary
Glucose and Lactate Monitoring Across the Rat SleepWake Cycle
waking triggered by a water puff (AW, −32 %), slow-wave sleep (SWS, +13 %), and paradoxical sleep (PS, −11 %). To prepare lactate biosensors, CFMEs were coated with lactate oxidase. These sensors, combined with DNPV measurements, showed a linear response
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