南京万木春生物科技有限公司

简介 动物用小鼠T细胞(Th1)进行免疫接种。 脾细胞与NS-1骨髓瘤细胞融合。 在活化的小鼠T细胞上检测到表达的一个 39KDa抗体蛋白。其重要性在于可为生命科学和生物技术领域的研究工作提供标准化的实验细胞资源及相关技术支 撑。

完全培养基配置 MEM培养基；10%胎牛血清；1%双抗 简介 CCD-18Co is a cell line exhibiting fibroblast morphology that was isolated from the normal colon tissue of a 2.5-month-old, Black, female. 形态 成纤维细胞 生长特征 贴壁生长

简介 该细胞系来源于一雌性中国仓鼠的肺组织。1970年由Koyama等建立。广泛应用于化学物质诱导的染色体畸变检测。 形态 成纤维细胞样 生长特征 贴壁生长

完全培养基配置 he base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006).To make the complete growth medium ，add the following components to the base medium:extra 10 mM HEPES (for a final conc. of 25 mM)10 ng/ml cholera toxin0.005 mg/ml insulin0.005 mg/ml transferrin100 ng/ml hydrocortisone20 ng/mL human recombinant EGF (Thermo Fisher PHG0311)Fetal Bovine Serum ， 10% final conc (ATCC 30-2020)

完全培养基配置 The base medium for this cell line is OptiMEM 1 Reduced Serum Medium (Gibco Catalog No. 31985). To make the complete growth medium, add the following components to the base medium: 20 mM HEPES 10 mM GlutaMAX (Gibco Catalog No. 35050) 10 ng/mL Epidermal Growth Factor (EGF) fetal bovine serum (FBS) to a final concentration of 4%