【Abstract】Objective:Toinvestigatetheeffectof plateletandendothelialcelladhesionmolecule1(PECAM1)ex-pressiononmigrationandinvasionabilityingastrointestinal stromaltumor(GIST)cells.Methods:Thetarget genewasscreenedbasedonGISTsingle -celltranscriptomesequencingdatabyRlanguage.Cellsweretransfectedwith and onco-vaccines. The anti-PD-1 checkpoint inhibitors nivolumab and pembrolizumab have been FDA approved as a second line treatment in patients who progressed or are intolerant to Sorafenib. To build up on the success of PD-1 monotherapy, combinatorial regimens with PD-1/PD-L1 inhibitors plus VEGF targeted agents have shown positive results in various