【共享】关于RNAi优化
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Optimizing RNA interference for application in mammalian cells
来源：
http://www.biochemj.org/bj/imps_x/pdf/BJ20040260.pdf
是一篇34页的综述，自己感觉不错就推荐给大家
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Abstract
Over the last two years the scientific community has rapidly embraced novel technologies that allow gene silencing in vertebrates. Ease of application, cost effectiveness and the possibilities for genome-wide reverse genetics have quickly turned this approach into a widely accepted, almost mandatory asset for a self-respecting lab in life sciences. This review discusses some of the recent technological developments that allow the application of RNAi in mammalian cells. In addition, the advantages of applying RNA interference to study cell cycle events and the emerging approaches to
perform mutational analysis by complementation in mammalian cells are evaluated. In addition, common pitfalls and drawbacks of RNA interference will be reviewed, as well as the possible ways to get around these shortcomings of gene silencing by small interfering RNA.
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来源：
http://www.biochemj.org/bj/imps_x/pdf/BJ20040260.pdf
是一篇34页的综述，自己感觉不错就推荐给大家
上传不了，自己下吧
Abstract
Over the last two years the scientific community has rapidly embraced novel technologies that allow gene silencing in vertebrates. Ease of application, cost effectiveness and the possibilities for genome-wide reverse genetics have quickly turned this approach into a widely accepted, almost mandatory asset for a self-respecting lab in life sciences. This review discusses some of the recent technological developments that allow the application of RNAi in mammalian cells. In addition, the advantages of applying RNA interference to study cell cycle events and the emerging approaches to
perform mutational analysis by complementation in mammalian cells are evaluated. In addition, common pitfalls and drawbacks of RNA interference will be reviewed, as well as the possible ways to get around these shortcomings of gene silencing by small interfering RNA.
如有人不能下载，请pm不要跟贴
