Screening RNA-Binding Libraries Using a Bacterial Transcription Antitermination Assay

A number of genetic assays have recently been developed for detecting RNA-protein interactions, several of which have been applied to screen cDNA or combinatorial libraries for RNA-binding peptides and proteins (1 –5 ) (see Chapters 13 and 15 ). These methods are useful for understanding the mechanisms of RNA-peptide and RNA-protein interactions, and may provide tools for designing compounds of therapeutic interest.