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        请教CASPASE 3抗体的选择?急

        丁香园论坛

        5030
        我准备做凋亡过程中CASPASE 3的变化,用WESTERN检测。我准备买SANTA CRUZ的抗体。有两种,一种是单抗,另一种是多抗。具体如下:
        1.caspase-3 (E-8): sc-7272
        SOURCE
        Caspase-3 (E-8) is a mouse monoclonal IgG2a antibody
        corresponding to amino acids 1-277 representing the full length
        precursor form of caspase-3 (also designated CPP32) of human
        origin.
        SPECIFICITY
        Caspase-3 (E-8) is recommended for the detection of caspase-
        (also designated CPP32) of human origin by Western blotting,
        immunoprecipitation, immunohistochemistry and FCM.
        2.caspase-3 (H-277): sc-7148
        SOURCE
        Caspase-3 (H-277) is a rabbit polyclonal antibody raised against a
        recombinant protein corresponding to amino acids 1-277
        representing the full length precursor form of caspase-3 of human
        origin.
        SPECIFICITY
        Caspase-3 (H-277) is recommended for the detection of p11, p17
        and p20 subunits and the full length precursor of caspase-3 (also
        designated CPP32) of mouse, rat and human origin by Western
        blotting, immunoprecipitation and immunohistochemistry; may
        cross-react with other cystein proteases.
        希望各位帮忙!!我用的是细胞系,来源是人的。
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