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        (请教)抗体的选择

        丁香园论坛

        3086
        请教前辈,我要做westernblot及免疫组化,抗原是人源性的PEDF,实验动物是大鼠,以下有两种santa cruz公司的抗体,source及specificity的说明不同,请问我选用哪一种更为适合,两者有何区别阿?谢谢指教!!!!

        SOURCE
        PEDF (H-125) is a rabbit polyclonal antibody raised against a recombinant protein corresponding to amino acids 281-405 mapping near the carboxy terminus of PEDF (pigment endothelium-derived factor) of human origin.
        SPECIFICITY
        PEDF (H-125) is recommended for the detection of PEDF (pigment endothelium-derived factor) of mouse, rat and human origin by Western blotting, immunoprecipitation and immunohistochemistry. Recommended dilution for Western blot analysis: 1:200-1:1000. Recommended starting dilution: 1:200.

        S O U R C E
        PEDF (I-15) is an affinity purified goat polyclonal antibody raised against a peptide mapping within an internal region of PEDF (pigment endothelium-derived factor) of human origin.
        S P E C I F I C I T Y
        PEDF (I -15) is recommended for detection of precursor and matu re PEDF of mou se, ra t an d human origin by Western blotting, immunoprecipitation and immunohistochemistry. Recommended dilution for Western blot analysis: 1:100-1:1000. Recommended starting dilution: 1:100.
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