【共享】RNAi实验的网站 This Caenorhabditis elegans RNAi feeding library
丁香园论坛
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这个是应用feeding的方法在线虫中应用RNAi，据研究称这是应用于线虫高通量RNAi最佳的方法。不过这个library的价格不知道怎样，不知道国内有没有买到这个library的实验室，如果有不知道分享价格如何？我拟使用该方法进行线虫基因组范围内的RNAi，哪位有这方面的经验能否赐教一二 谢谢。
http://www.hgmp.mrc.ac.uk/geneservice/reagents/products/descriptions/Celegans.shtml
内容如下
C. elegans RNAi library
This Caenorhabditis elegans RNAi feeding library provided by MRC geneservice was constructed by Julie Ahringer's group at the The Wellcome CRC Institute, University of Cambridge, Cambridge, England. .
C. elegans genomic fragments were PCR amplified using Research Genetics GenePairs, cloned into the EcoRV site of vector L4440 from Timmons and Fire (Nature, 395, 854) and transformed into bacterial strain HT115 (Gene, 263, 103-112) as described (Nature 408, 325-330). The whole genome library consists of 16,757 bacterial strains, which cover 87% of C. elegans genes.
Bacterial strains carry the GenePairs name, which usually, but does not always correspond to a predicted C. elegans gene name. A current mapping of GenePair to gene can be found in WormBase (http://www.wormbase.org). GenePairs primer sequences are available at http://cmgm.stanford.edu/~kimlab/primers.12-22-99.html.
There is also a "C.elegans Finder" tool available which allows you to find the primer sequences for any given GenePair Name in addition to the location of that bacterial strain in the HGMP 384-well plates. The complete C.elegans RNAi database can be downloaded by right clicking here
The libraries is available by individual chromosome sets (I, II, III, IV, V and X) from MRC geneservice as frozen glycerol stocks of bacterial strains arrayed in 384 well plates or as individual bacterial strains (clones).
http://www.hgmp.mrc.ac.uk/geneservice/reagents/products/descriptions/Celegans.shtml
内容如下
C. elegans RNAi library
This Caenorhabditis elegans RNAi feeding library provided by MRC geneservice was constructed by Julie Ahringer's group at the The Wellcome CRC Institute, University of Cambridge, Cambridge, England. .
C. elegans genomic fragments were PCR amplified using Research Genetics GenePairs, cloned into the EcoRV site of vector L4440 from Timmons and Fire (Nature, 395, 854) and transformed into bacterial strain HT115 (Gene, 263, 103-112) as described (Nature 408, 325-330). The whole genome library consists of 16,757 bacterial strains, which cover 87% of C. elegans genes.
Bacterial strains carry the GenePairs name, which usually, but does not always correspond to a predicted C. elegans gene name. A current mapping of GenePair to gene can be found in WormBase (http://www.wormbase.org). GenePairs primer sequences are available at http://cmgm.stanford.edu/~kimlab/primers.12-22-99.html.
There is also a "C.elegans Finder" tool available which allows you to find the primer sequences for any given GenePair Name in addition to the location of that bacterial strain in the HGMP 384-well plates. The complete C.elegans RNAi database can be downloaded by right clicking here
The libraries is available by individual chromosome sets (I, II, III, IV, V and X) from MRC geneservice as frozen glycerol stocks of bacterial strains arrayed in 384 well plates or as individual bacterial strains (clones).
