抗体抗原实验

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。高质量的抗体则可通过提取纯化而获得。在制备方法上，宜 ...

同位素标记是一种重要的抗体标记技术，�977年Yalow等在放射免疫测定法所作突出贡献而获得医学和生理学诺贝尔奖。McAb标记同位素后除应用于放射免疫测定外，还可用于竞争结合试验、体内肿瘤定位等。　　(一)　原理　　氯胺�即对甲苯碘氯胺钠，在水溶液中生成次氯酸，为一种温和氧化剂，当加入到有蛋白质和碘的弱碱水溶液中，可将I２氧化为I＋并结合到氨基酸上。如标记条件温和，标记后的McAb仍具有良好的结合 ...

・ Histological techniques (William H. Heidcamp)Very detailed guide to histological techniques like fixation dehydration embedment and subsequent sectioning. ・ 3-Aminopropyltriethoxy ...

Tissue Preparation for Methy Methacrylate Embedding Methyl Methacrylate Embedding Protocols (Energy Beam Sciences Inc.)General protocol for plastic embedding technique Sectioning and Mounting Protoc ...

Fixative Preparation 10% Formal Saline Preparation (Nottingham Pathology Lab) 4% Paraformaldehyde Preparation (Nottingham Pathology Lab) Fixatives (XMMR) Staining SolutionCarazzi's Haematoxylin (No ...

（一） 原理 免疫胶体金技术是以胶体金作为示踪标志物应用于抗原抗体的一种新型的免疫标记技术。胶体金是由氯金酸（HAuCl4）在还原剂如白磷、抗坏血酸、枸橼酸钠、鞣酸等作用下，聚合成为特定大小的金颗粒，并由于静电作用成为一种稳定的胶体状态，称为胶体金。胶体金在弱碱环境下带负电荷，可与蛋白质分子的正电荷基团形成牢固的结合，由于这种结合是静电结合，所以不影响蛋白质的生物特性。胶体金除了与蛋白质结合 ...

Laser Capture Microdissection (LCM) Introduction to LCM (BJMU) Preparation LCM and RNA/DNA extraction of Frozen Tissue Sections (NIH Laser Capture Microdissection Core Lab) Preparation and LCM of P ...

・ Histological techniques (William H. Heidcamp)Very detailed guide to histological techniques like fixation dehydration embedment and subsequent sectioning. ・ Cryosectioning (House ...

Microwave Processing Techniques for Microscopy (Energy Beam Sciences Inc.)Introduction to microwave technique and detailed protocol for its applications Special Stains Using the Microwave (Energy B ...

・ Double Peroxidase (HRP) Immunohistochemical Labeling of Trypsin-Sensitive Antigens (KPL)・ Immunohistochemistry (Tyner lab)This is a basic immunohistochemistry protocol. Protocols for ...

・ Tips and hints for the storage of antibodies (Synaptic Systems) ・ Purification of IgG Using Protein A- or Protein G-Agarose (KPL) ・ Production of Monoclonal Antibody (Chisho ...

Making Antibody ・ Production of Polyclonal Antibody in Rabbit (Walter Steffen)Provides detailed protocol for immunizatioin bleeding procedure obtaining serum precipitation of IgG.・ GST ...

Antibody PurificatioinPurification of IgG Using Protein A- or Protein G-Agarose (KPL) Purifying Antibodies (Perkin-Elmer) Precipitation MethodsProtein A or Protein G Columns or BeadsAntigen Columns ・ ...

Biotinylation of Antibody ( Contributed by Nanci Donacki) Antibody labeling (House Ear Institute)Labeling protocols using affinity markers. Coupling Colloidal Gold to Protein (Walter Steffen) Gold ...

Immunofluorescence Technique (Spector Lab)protocol for immunofluorescence on cells Immunofluorescence Protocol (Walter Steffen) Preparation of cover glasses Cell culture Fixation protocol Methanol f ...

Gangliosides ELISA protocol (Contributed by pingsunjim)This protocol can be used for detection of gangliosides.Specific antibodies to gangliosides are added to cells and allowed to bind to the cell su ...

・ Lymphocyte Transformation (Donis-Keller lab)Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from anticoagulated venous blood are isolated by layering ont ...

Introduction to Immunocytochemistry (House Ear Institute)A brief overview of common available methods. BrDU Immunocytochemistry using peroxidase and DAB for rat tissues (Contributed by Shyam Laxmina ...

　ELISA试剂的评价（evaluation）分两个方面：一是试剂本身的质量评价，符合一定要求后才能生产供应；一是在临床应用中效果的评价。以肝炎ELISA诊断试剂为例，首先必须通过中国药品生物制品检定，以得到生产的许可。检定内容除包装、标签、说明书等外，对试剂的性能，如特异性、灵敏度、精密度和线性等均需逐项检定，通过对一系列参比品的检测，结果符合要求者才为合格。ELISA试剂的临床质量评价是用该试 ...

从1949年美国College of American Pathologists（简称CAP）首先开始研究临床实验室室内质量控制（简称质控）问题，美国学者Levery和Jenning于1950年发表第一篇关于使用质控图的实验室室内质控，临床检验实验室的室内质控工作正式拉序幕。　　到70年代，实验室质量控制进入一个新的阶段--全面质量管理，推行Good Laboratory Parctice（简称G ...