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        抗体纯化

        互联网

        2050

        Antibody Purificatioin

        • Purification of IgG Using Protein A- or Protein G-Agarose (KPL)
           
        • Purifying Antibodies (Perkin-Elmer)

        Precipitation Methods
        Protein A or Protein G Columns or Beads
        Antigen Columns

        ・         Affinity Purification of Antibodies from Crude Serum (PMCI)

        ・         Affinity Purification of Antibody (Walter Steffen)

          • Coupling procedure
          • Affinity purification
          • Absorption of antibodies to acetone powder
          • Production of acetone powder
          • Extraction of antibodies from egg yolk
          • Purification and cleavage of murine monoclonal antibodies
          • IgG purification with protein G
          • Digest of IgG with papain
              

        ・         Affinity Purification of Antibodies (Vesicle Trafficking)

        ・         Affinity Purification of Antibodies (XMMR)
        protocol in great detail, including column preparation, wash and more...

        ・         Protein G Purification of Antibodies (Contributed by Nanci Donacki) 

        ・         Protein A Purification of Antibody (Contributed by Nanci Donacki) 

        • Coupling Antibodies to Protein A/G (Breeden Lab)
            
        • Coupling Peptides to Resin for Affinity Purification (Mitshison Lab)
            
        • Purification of Anti Peptide Antibody (Mitshison Lab) 
              
        • Antibody Purification (Mike A. Dyer) 
          This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography.

        Column Preparation

        • Making Affinity Columns (XMMR)

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