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        抗体标记方法

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        3899

        • Biotinylation of Antibody ( Contributed by Nanci Donacki )
        • Antibody labeling (House Ear Institute)
          Labeling protocols using affinity markers.
        • Coupling Colloidal Gold to Protein (Walter Steffen)
          • Gold labelling of rabbit IgG
          • Preparation of enzyme gold complex
          • Coupling of Nanogold to IgG
        • Production of Colloidal Gold (Walter Steffen)
        • Protein (Antibody) Fluorescent Labeling (Mario Roederer)
          Detailed protocol for conjugation of proteins (principally monoclonal antibodies) to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V). Procedures for the conjugation of immunoglobulins are provided for the following molecules:
          • Fluorescein (FITC)
          • Phycoerythrin (PE)
          • Cy5PE
          • Cy7PE
          • Texas Red (TR)
          • Allophycocyanin (APC)
          • Cy5
          • Cy7APC
          • Cascade Blue
          • Biotin

        In addition, protocols for conjugation of Annexin V to the following molecules are given as models for fluorescent conjugation of non-immunoglobulin proteins.

          • FITC
          • PE
          • TR-BSA
          • APC
          • Cascade Blue

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