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        组织学――组织制备

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        1681

         

                 Histological techniques (William H. Heidcamp)
        Very detailed guide to histological techniques, like  fixation, dehydration, embedment and subsequent sectioning.
          

                 3-Aminopropyltriethoxysilane Treatment of Slides (Nottingham Pathology Lab)
          

                 Paraffin-Embedded Tissue for LCM (Arcturus)
        Paraffin embedding is a process in which fixed (formalin or other fixative) is infiltrated and then blocked out in paraffin to stabilize it for long-term storage and easy sectioning...

           

                 Wax embedding/sectioning (Klymkowsky Lab)
          

        • Chrome Gelatinised Slides and Coverslips (Nottingham Pathology Lab)
             
        • Decalcification (Nottingham Pathology Lab)
          Many surgical specimens contain calcified areas which need to be decalcified before processing and sectioning. This is the protocol used for this purpose
            
        • De-Paraffinization of Tissue Sections (Arcturus)
          Remove paraffin from section for tissue preparation such as nucleic acid extraction.
             
        • DNA Extraction From Paraffin Section (Arcturus)
          This extraction method has been used for measuring loss of heterozygosity (LOH), dideoxy fingerprinting (DDF), clonality analysis (chromosome X inactivation) and direct sequencing of PCundefined products for single base mutational analysis. Extractions are typically performed on 500-1000 captured cells.
            
        • RNA Extraction from Paraffin Section (NIH Protocol) (Arcturus)
          A Protocol for extracting RNA from paraffin embedded tissue.
            
        • Preparation of Frozen Tissue for LCM (Arcturus)
            
        • White Blood Cell Cytospin (Arcturus)

         

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