免疫组织化学

互联网2013-09-06

2021

・ Double Peroxidase (HRP) Immunohistochemical Labeling of Trypsin-Sensitive Antigens (KPL)

・ Immunohistochemistry (Tyner lab)
This is a basic immunohistochemistry protocol. Protocols for immunohistochemistry usr/localy widely, due to the differences between antigens and their recognition by antibody. For success it is crucial to try a number of different techniques and compare the results.

・ Treptavidin/Beta-Galactosidase Immunohistochemical Labeling Procedure (KPL)

Immunohistochemical Staining of Floating Thick Sections (10-100 microns) (KPL)
General Protocol for staining thick sections
Immunohistochemical Staining for Formalin-Fixed, Paraffin-Embedded Tissues (LEP)
This staining procedure is for tissues fixed in 10% neutral buffered formalin and embedded in paraffin
Immunohistochemical Stain-Alkaline Phosphatase (APAAP) Technique (Nottingham Pathology Lab)
Immunohistochemisty-Enzymatic Protocol (R&D)
General protocol including details for sample preparation, tissue staining and trouble shooting
Immunohistochemisty-Fluorescence Protocol (R&D)
General protocol
Immunohistochemistry of Mouse Frozen Tissues (eBioscience)
Immunofluorescence- Whole Mount (Karl Clark)
Immunohistochemistry allows visualization of antigens (usually proteins) within an embryo. Typically, a primary antibody binds specifically to an antigen (e.g. Luciferase); then a secondary antibody conjugated to a flourochrome or enzyme is bound to the heavy chain constant region of the primary antibody with specificity to the species of origin of the primary (e.g. anti-rabbit conjugated to Rhodamine
Immunohistochemistry on Whole-Mount Embryos (Vesicle Trafficking)
Wholemount Staining of Embryos (XMMR)
Beta-gal Staining- Whole Mount (Karl Clark)
Beta-gal staining allows identification of embryonic tissues/cells expressing lacZ marker protein by development of pigmented (blue) product in the presence of lacZ enzymatic activity.
X-gal & AP Staining Protocol (Cepko Lab)
Very detailed protocol with background information and several applications.
Reducing Background Staining in Immunohistochemistry (KPL)
Histochemical Visualization of Mycoplasma paratuberculosis (KPL)
Solutions and Calculations for Immunohistochemistry (KPL)
A semi-permenant mounting medium for immunofluorescence microscopy (House Ear Institute)
A recipe for Moviol,
an inexpensive mounting medium that is easy to prepare.
Microwave Immunohistochemistry( Energy Beam Sciences, Inc.)
Introduction to microwave immunohistochemistry technique and its applicaiton protocol
Antigen Retrieval : for immunohistochemistry (Mike A. Dyer)
Dissociated Cell Staining : immunohistochemistry (Mike A. Dyer)