- ￥4800
- Tanda
- 美国
- TD-26326
- 2025年10月25日
- ELISA, WB, IHC
- Mouse
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide from the internal region of CTCF which includes the mutation of R339W, human origin.
- 亚型：
lgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白
- 目录编号：
TD-26326
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
ELISA, WB, IHC
- 浓度：
1mg/ml
- 靶点：
R339W
- 抗体英文名：
Anti-CTCF(R339W) Mouse Monoclonal Antibody
- 抗体名：
CTCF(R339W)
- 规格：
100 μL
CTCF(R339W)
| Cat. #: TD-26326 |
| Gene Symbol: CTCF; MRD21 |
| Description: Anti-CTCF(R339W) Mouse Monoclonal Antibody |
| Background: Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, recombination and regulation of chromatin architecture. |
| Immunogen: A synthetic peptide from the internal region of CTCF which includes the mutation of R339W, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes R339W mutant, but not wild type CTCF of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Western blot:
Western blot analysis of recombinant CTCF (R339W) and wild type proteins. Purified His-tagged CTCF (R339W) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-CTCF(R339W) monoclonal antibody (Cat. #TD-26326). Immunofluorescence:
Immunofluorescence of cells expressing CTCF proteins with Anti-CTCF(R339W) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTCF (WT) plasmid (left column) or pCDNA3-GFP-CTCF (R339W) plasmid (right column), then fixed and stained with Anti-CTCF(R339W) monoclonal antibody (Cat. #TD-26326). |
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文献和实验Generation of Poly(ADP-ribosyl)ation Deficient Mutants of the Transcription Factor, CTCF
to produce such mutants, demonstrated by the example of CTCF, a transcription factor. To achieve this in our study, the protein domain modified by PARylation was mapped and the amino acids, which can be potentially PARylated, selected. Mutations
：Buffer、体系、孵育时间、spike-in DNA 量以及建库制备和纯化过程。 图 6：不同细胞量 mESCs 的 uliCUT&RUN 数据结果[4] 将不同细胞量 mESC 的 uliCUT&RUN 数据中的 CTCF 和 H3K4me3 测序结果与已有文献发表的 ChIP-seq 数据进行 read 密度可视化比较，发现 10 个细胞起始量的测序结果可以展现出类似 50 万细胞量的结合位点富集热图。其中 CTCF 的结合区更为狭窄集中，而 H3K4me3 则占据相对宽的区段。 ✦
Secondary structure of prion mRNA
. R. Soc. Lond. Biol. 339, 287294. Joshi, R. L., Joshi, S., Chapeville, F. & Haenni, A. L. (1983). tRNA-like structures of plant viral RNAs: confor-mational requirements for adenylation and aminoa-cylation. EMBO J. 2, 11231127. Kellings, K., Meyer
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