- ￥2600
- Tanda
- TD-21202
- 美国
- 2026年03月18日
- ELISA, WB, IHC
- 兔
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉天德
- 库存：
999
- 靶点：
ErbB2
- 级别：
科研级
- 目录编号：
TD-21202
- 克隆性：
多克隆
- 抗原来源：
蛋白
- 保质期：
24个月
- 抗体英文名：
Anti-ERBB2 Rabbit polyclonal antibody
- 抗体名：
ErbB2 Polyclonal AntibodyErbB2 Polyclonal Antibody [TD-21202]
- 标记物：
FITC
- 宿主：
兔
- 适应物种：
脊椎动物
- 免疫原：
A synthetic peptide from the central of ErbB2 protein.
- 亚型：
lgG
- 形态：
液体
- 应用范围：
ELISA, WB, IHC
- 保存条件：
-20℃
- 浓度：
0.2mg/ml
- 规格：
100μl
ErbB2 Polyclonal Antibody [TD-21202]
| Cat. #: TD-21202 |
| Gene Symbol: ERBB2/HER2/Neu |
| Description: Anti-ErbB2 Rabbit Polyclonal Antibody |
| Background: The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis. |
| Immunogen: A synthetic peptide from the central of ErbB2 protein. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:500-1:5000 WB: 1:200-1:2000 IHC: 1:20-1:200 |
| Concentration: 0.2 mg/ml |
| Host Species: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: IgG |
| Purity: Purified from serum |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes ErbB2 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. |
Western blot:  Western blot analysis of ErbB2 protein. Purified full length His-tagged ErbB2 protein (Cat. #) was blotted with Anti-ErbB2 rabbit polyclonal antibody (Cat. #TD-21202).  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Anti-ErbB2 polyclonal antibody (Cat. #TD-21202). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4 °C. A HRP-conjugated Goat anti-Rabbit IgG (1:50) was used as secondary antibody. |
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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