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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide from the internal region of CTCF which includes the mutation of K344E, human origin.
- 亚型:
lgG
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
单克隆
- 标记物:
FITC
- 适应物种:
脊椎动物
- 保质期:
24个月
- 抗原来源:
蛋白
- 目录编号:
TD-26324
- 级别:
科研级
- 库存:
999
- 供应商:
武汉天德
- 宿主:
Mouse
- 应用范围:
ELISA, WB, IHC
- 浓度:
1mg/ml
- 靶点:
K344E
- 抗体英文名:
Anti-CTCF(K344E) Mouse Monoclonal Antibody
- 抗体名:
CTCF(K344E)
- 规格:
100 μL
CTCF(K344E)
| Cat. #: TD-26324 |
| Gene Symbol: CTCF; MRD21 |
| Description: Anti-CTCF(K344E) Mouse Monoclonal Antibody |
| Background: Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, recombination and regulation of chromatin architecture. |
| Immunogen: A synthetic peptide from the internal region of CTCF which includes the mutation of K344E, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes K344E mutant, but not wild type CTCF of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Western blot:
Western blot analysis of recombinant CTCF (K344E) and wild type proteins. Purified His-tagged CTCF (K344E) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-CTCF(K344E) monoclonal antibody (Cat. #TD-26324). Immunofluorescence:
Immunofluorescence of cells expressing CTCF proteins with Anti-CTCF(K344E) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTCF (WT) plasmid (left column) or pCDNA3-GFP-CTCF (K344E) plasmid (right column), then fixed and stained with Anti-CTCF(K344E) monoclonal antibody (Cat. #TD-26324). |
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文献和实验Generation of Poly(ADP-ribosyl)ation Deficient Mutants of the Transcription Factor, CTCF
to produce such mutants, demonstrated by the example of CTCF, a transcription factor. To achieve this in our study, the protein domain modified by PARylation was mapped and the amino acids, which can be potentially PARylated, selected. Mutations
:Buffer、体系、孵育时间、spike-in DNA 量以及建库制备和纯化过程。 图 6:不同细胞量 mESCs 的 uliCUT&RUN 数据结果[4] 将不同细胞量 mESC 的 uliCUT&RUN 数据中的 CTCF 和 H3K4me3 测序结果与已有文献发表的 ChIP-seq 数据进行 read 密度可视化比较,发现 10 个细胞起始量的测序结果可以展现出类似 50 万细胞量的结合位点富集热图。其中 CTCF 的结合区更为狭窄集中,而 H3K4me3 则占据相对宽的区段。 ✦
聚集成 CpG 岛(CCIs),50% 以上的基因启动子都含 CCIs,一般都集中在转录起始位点。甲基的存在导致了转录过程终止,从而使这些基因沉默。其机制主要与转录因子 CTCF(CCCTC binding factor)蛋白和甲基 CpG 结合蛋白(MBP)有关。CTCF 可以控制染色质三维结构的改变,促进或抑制不同位点的基因表达,但 CTCF 不能与甲基化CpG 结合,MBP 含有甲基化 CpGs 结合域,同时能与组蛋白去乙酰化酶(HDAC)结合,可重构染色质并抑制转录。 图
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