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AKT1(E49K)

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  • ¥4800
  • Tanda
  • 美国
  • TD-26137
  • 2025年10月28日
  • ELISA, WB, IHC
  • Mouse
  • 脊椎动物
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      A synthetic peptide from the internal region of AKT1 which includes the mutation of E49K, human origin.

    • 亚型

      lgG

    • 形态

      液体

    • 保存条件

      -20℃

    • 克隆性

      单克隆

    • 标记物

      FITC

    • 适应物种

      脊椎动物

    • 保质期

      24个月

    • 抗原来源

      蛋白

    • 目录编号

      TD-26137

    • 级别

      科研级

    • 库存

      999

    • 供应商

      武汉天德

    • 宿主

      Mouse

    • 应用范围

      ELISA, WB, IHC

    • 浓度

      1mg/ml

    • 靶点

      E49K

    • 抗体英文名

      Anti AKT1(E49K) Mouse Monoclonal Antibody

    • 抗体名

      AKT1(E49K)

    • 规格

      100 μL

    AKT1(E49K)

    Cat. #:  TD-26137
    Size:  100 µl
    Gene Symbol:  AKT1; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
    Description:   Anti-AKT1(E49K) Mouse Monoclonal Antibody
    Background:   AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinases, which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Its function is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. There are evidences that mutation or abnormal expression in AKT1 is associated with cancers.
    Immunogen:   A synthetic peptide from the internal region of AKT1 which includes the mutation of E49K, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host Species:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Preservative: No
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes E49K mutant, but not wild type AKT1 of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
    Western blot: 

     产品细节图片1
    Western blot analysis of recombinant AKT1(E49K) and wild type proteins. Purified His-tagged AKT1(E49K) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-AKT1(E49K) monoclonal antibody (Cat. #TD-26137).
    Immunofluorescence:

      产品细节图片2
    Immunofluorescence of cells expressing AKT1 proteins with Anti-AKT1(E49K) antibody. HEK293T cells were transfected with pCDNA3-GFP-AKT1 (WT) plasmid (left column) or pCDNA3-GFP-AKT1(E49K) plasmid (right column), then fixed and stained with Anti-AKT1(E49K) monoclonal antibody (Cat. #TD-26137).

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