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文献和实验Vybrant® DyeCycle Green and Orange Stains
DyeCycle™ Staining Protocol This basic protocol is optimized using Jurkat cells suspended in complete medium (RPMI/10% fetal bovine serum) and stained with Vybrant® DyeCycle™ stain at 37?C. Remove the Vybrant® DyeCycle™ Green stain
the elutes (Turbo DNA-free™, Ambion) and then perform an RT reaction (+/- RT) followed by PCR with primers specific for the siRNA targeted promoter (Figure 2). Reviewer Comments 1.Reviewed by: Jeannie Lee, Department of Genetics (and Pathology), Harvard
Vybrant® DyeCycle Violet Stain
This basic protocol is optimized using Jurkat cells suspended in complete medium (RPMI/10% fetal bovine serum) and stained with Vybrant® DyeCycle™ Violet stain at 37?C. Remove the Vybrant® DyeCycle™ Violet stain from the refrigerator and allow
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