- ￥3000
- Thermo Fisher Scientific
- N8010535
- 2025年07月13日
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上海玉博生物科技有限公司
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文献和实验Single tube confirmation PCR protocol
Polymerase. - Add 35 µl of the PCR mix to each of the 20 PCR tubes that already contain the 15 µl of primers and template. 110 µl 5 µl 10 x Taq buffer(see below) 11 µl 0.5 µl 20 mM dNTP's (0.2 mM) 11 µl 0.5 µ
Reverse transcription (RT) Materials required RT-PCR kit (Gibco) Primers (300 nM each) PCR thermocycler Microfuge Microfuge 0.5 ml thin walled PCR tubes Thermocycler Ð Caution
Single tube confirmation PCR protocol
The following colony PCR protocol has been designed to be performed in individual reaction tubes. We usually test three colonies from each transformation along with a wild-type control. A series of five different PCR tests are performed
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