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文献和实验Vybrant® DyeCycle Green and Orange Stains
DyeCycle™ Staining Protocol This basic protocol is optimized using Jurkat cells suspended in complete medium (RPMI/10% fetal bovine serum) and stained with Vybrant® DyeCycle™ stain at 37?C. Remove the Vybrant® DyeCycle™ Green stain
to avidin magnetic beads (Dynal™). The final elutes, following the binding of the biotin linked RNAs with the avidin magnetic beads and washing, were then DNAse, RNAse H or RNAse A treated, RT (BioRad™ iScript) reacted and PCR amplified. References 1.Kim
Isolate It All:siRNA • miRNA • Total RNA • Native Protein
Figure 2. Analysis of Total RNA Isolated with the mir Vana ™ PARIS ™ Kit. Total RNA was isolated from 1 x 106 HeLa cells with the indicated kit as per protocol. (A) 1 µg of total RNA was resolved on a 15% denaturing acrylamide gel and stained
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