VspI内切酶北京品牌

特别提示：包括VspI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：VspI内切酶
英文名称：VspI
产品货号：GS2128
产品规格：1kU

储存条件：冷冻（-20℃）

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	AT↑TAAT
来源	E.coli strain,that carries the cloned gene VspI from Vibrio species 343
Assayed on	Lambda DNA
Optimal buffer	W?(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.)
Optimal temperature	37℃
Storage conditions	10mMTris-HCl(pH 7.6);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol.Store at -20℃.
Ligations	After 10-fold overdigestion with enzyme 70%of the DNA fragments can be ligated.Of these,90%can be recut.In the presence of 10%PEG ligation is better.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃
Methlation effect	Blocked by ATTAmAT methylation.
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends	AseI,AsnI,PshBI

我公司销售的VspI内切酶北京品牌，VspI限制性内切酶质量上佳，价格普惠，欢迎广大新老客户踊跃订购。