BstMBI内切酶北京现货

特别提示：包括BstMBI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：BstMBI内切酶
英文名称：BstMBI
产品货号：GS2119
产品规格：100U

储存条件：冷冻（-20℃）

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(dam-)in 1hourat 65℃ in a total reaction volume of 50μl.
Recognition sites	↑GATC
来源	Bacillus stearothermophillus MB
Assayed on	Lambda DNA(dam-)
Optimal buffer	O?(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.)
Optimal temperature	65℃
Storage conditions	10 mM Tris-HCl(pH 7.5);50mMKCl;0.1 mM EDTA;200 μg/ml BSA.Store at -20℃.
Ligations	After 5-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 65℃
Methlation effect	Blocked by overlapping Dam methylation(GmATC):?GATC?.|Not blocked by CG methylation.|Not cut hemimethylated site:5- G(6mA)TC-3/3-CTAG-5
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	BfuCI,BscFI,Bsp143I,BssMI,BstENII,BstKTI^,DpnI^,Kzo9I,MalI^,MboI,NdeII^,Sau3AI

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