Phospho-mTOR (Ser2448) Antibod

2971:

Western Blotting

Phospho-mTOR (Ser2448) Antibody detects endogenous levels of mTOR only when phosphorylated at Ser2448.

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human mTOR. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from 293 cells (starved for 16 hours) untreated or EGF-treated (100 ng/ml), using Phospho-mTOR (Ser2448) Antibody (upper) or control mTOR Antibody #2972 (lower).

The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).

1. Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22.
2. Brown, E.J. et al. (1994) Nature 369, 756-8.
3. Sabatini, D.M. et al. (1994) Cell 78, 35-43.
4. Gingras, A.C. et al. (2001) Genes Dev 15, 807-26.
5. Dennis, P.B. et al. (2001) Science 294, 1102-5.
6. Fang, Y. et al. (2001) Science 294, 1942-5.
7. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
8. Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.
9. Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.

• Bolster, D. R. et al. (2002) AMP-activated protein kinase suppresses protein synthesis in rat skeletal muscle through down-regulated mammalian target of rapamycin (mTOR) signaling. J. Biol. Chem. 277, 23977-23980. Applications: Western Blotting
• Suzuki, A. et al. (2004) ARK5 is a tumor invasion-associated factor downstream of Akt signaling. Mol. Cell. Biol. 24, 3526-3535. Applications: Western Blotting
• Prieto, A.L. et al. (2007) Neuroscience 150, 319-34. Applications: Western Blotting
• Shavlakadze, T. et al. (2010) J Cell Sci , . Applications: Western Blotting
• Hasumi, Y. et al. (2009) Proc Natl Acad Sci U S A 106, 18722-7. Applications: Western Blotting
• Fei, Z. et al. (2011) J Biol Chem 286, 27761-8. Applications: Western Blotting

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• 2974 Phospho-mTOR (Ser2481) Antibody
• 2976 Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific)
• 2983 mTOR (7C10) Rabbit mAb
• 2972 mTOR Antibody
• 4517 mTOR (L27D4) Mouse mAb
• 2280 Raptor (24C12) Rabbit mAb
• 3274 GβL (86B8) Rabbit mAb
• 2691 PRAS40 (D23C7) XP^® Rabbit mAb
• 2114 Rictor (53A2) Rabbit mAb
• 9205 Phospho-p70 S6 Kinase (Thr389) Antibody
• 9644 4E-BP1 (53H11) Rabbit mAb
• 9964 mTOR Pathway Antibody Sampler Kit
• 9862 mTOR Substrates Antibody Sampler Kit
• 9864 mTOR Regulation Antibody Sampler Kit
• 1230 Phospho-mTOR (Ser2448) Blocking Peptide
• 1072 mTOR Blocking Peptide
• 9904 Rapamycin (FRAP/mTOR Inhibitor)
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.