- ￥5600
- GeneTex
- 美国
- GTX24816
- 2025年07月15日
- WB, ICC/IF, IHC-P, IP, IHC
- Rabbit
- Human, Mouse, Rat, Yeast, Drosophila, Bovine, Dog, Chicken, Pig, Xenopus
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide derived from the region of Src that contains tyrosine 416 (tyrosine 417 including the initiating methionine). The Tyr418 of human Src is equivalent to Tyr416 of chicken Src.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Yeast, Drosophila, Bovine, Dog, Chicken, Pig, Xenopus
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX24816
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP, IHC
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Src (phospho Tyr416)
- 抗体英文名：
Src (phospho Tyr416) antibody
- 抗体名：
Src (phospho Tyr416) 抗体
- 规格：
50 μl
WB analysis of samples using GTX24816 Src (phospho Tyr416) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
IHC-P analysis of human colon carcinoma tissue using GTX24816 Src (phospho Tyr416) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
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文献和实验Carrasco-Torres G et al., Oxid Med Cell Longev 2017 (PMID:28740570)
src 的产物 p60src 具有 TPK （酪氨酸蛋白激酶）活性，分子量约为 60KDa ，与细胞的增殖和转化有关。 Src 蛋白有四个结构域： （1） N 端十四烷基化位点是和膜结合的位点； （2） SH2 （ src homology region-2 ）结构域能和自身的或其它蛋白的磷酸化酪氨酸位点结合，传递信号。 （3） 416 位 Tyr 的自身磷酸化和细胞的转化活性有关。
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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