- ￥5300
- GeneTex
- 美国
- GTX54701
- 2025年07月15日
- WB
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A phospho specific peptide corresponding to residues surrounding Y418 of human SRC. The Tyr418 of human Src is equivalent to Tyr416 of chicken Src.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX54701
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Based on sequence homology, this antibody may cross with other Src family members such as YES1 , FYN proteins when phosphorylated at the equivalent sites.
- 抗体英文名：
Src (phospho Tyr416) antibody
- 抗体名：
Src (phospho Tyr416) 抗体
- 规格：
100 μl
WB analysis of COLO205 cell lysate using GTX54701 Src (phospho Tyr416) antibody.
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文献和实验Chang YX et al., Cancers (Basel) 2020 (PMID:33371405)
src 的产物 p60src 具有 TPK （酪氨酸蛋白激酶）活性，分子量约为 60KDa ，与细胞的增殖和转化有关。 Src 蛋白有四个结构域： （1） N 端十四烷基化位点是和膜结合的位点； （2） SH2 （ src homology region-2 ）结构域能和自身的或其它蛋白的磷酸化酪氨酸位点结合，传递信号。 （3） 416 位 Tyr 的自身磷酸化和细胞的转化活性有关。
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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