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- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
6month
- 英文名：
Mach1-T1 chemically Competent Cells
- 库存：
货源充足
- 供应商：
LSM Bio
- 规格：
0.1ml*10
Mach1-T1 chemically Competent Cells
Packing
Mach1-T1 Competent Cells锛?nbsp; 10μl/tube
pUC19锛坈ontrol vector锛?0ng/μl ) 10μl
Storage -80鈩冿紙6month锛?/p>
Mach1-T1 chemically Competent Cells Genotype
F- φ80(lacZ)ΔM15 ΔlacX74 hsdR(rK- mK+) ΔrecA1398 endA1 tonA
Mach1-T1 chemically Competent Cells Description
Mach1-T1 strains from the wild type non-K-12 E. Coli strain W transformation, is the growth rate of competent cells faster, on the plate of 8h visible clone deletion endonuclease (endA), improve the yield and quality of plasmid DNA; recombinant enzyme deficient (recA1398) to reduce the probability of homologous recombination fragment insertion, guarantee the stability of insertion DNA; lacZ M15 of the Mach1-T1 can be used by blue white screening; tonA resistance mutation gives Mach1-T1 strains on phage T1 and T5. The Mach1-T1 receptive cells were made by a special process, and the pUC19 plasmid was used to detect the conversion efficiency of >109cfu/ mu g DNA.
Caution
1.STORAGE AND HANDLING: Competent cells should be stored at–80°C. Storage at –20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above –80°C, even if they do not thaw.
2. This product is FOR RESEARCH USE ONLY!
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文献和实验TOP10 chemically competent cells
. See Bacterial Transformation for a more general discussion of other techniques. The Jesse ''464 patent describes using this buffer for DH5α cells. The Bloom04 patent describes the use of essentially the same protocol for the Invitrogen Mach 1 cells.
Preparing chemically competent cells
Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating
Standard Operating Procedures for T1-Phage Testing Assay
lawn, the lawn there will be lysed on incubation overnight. II. Materials and Equipments: DH10B (original glycerol stock) or DH10B cultured stock for phage test. DH10B-T1 phage infected cells (positive control).
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