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- LSM Bio
- CHC00089
- 2025年07月17日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
6month
- 英文名：
OrigamiB(DE3) chemically Competent Cells
- 库存：
货源充足
- 供应商：
LSM Bio
- 规格：
0.1ml*10
OrigamiB(DE3) chemically Competent Cells
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文献和实验TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
efficiency is sufficient, we use heat shock transformation and chemically competent cells. The choice of the E. coli host strain depends on the goal of the transformation. ·The transformation of a ligation mix should be done in a recA- cloning strain
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