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- LSM Bio
- CHC00079
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
6month
- 英文名：
TG1 chemically Competent Cells
- 库存：
货源充足
- 供应商：
LSM Bio
- 规格：
0.1ml*10
TG1 chemically Competent Cells
TG1 Chemically Competent Cell 100μl/tube
pUC19 (control vector锛?0pg/μl) 10μl
Storage at -80鈩冿紙6month锛?/p>
TG1 Chemically Competent Cell Genotype
[F´traD36 proAB lacIqZΔM15] supE thi-1Δ(lac-proAB)Δ(mcrB-hsdSM)5(rK–mK–)
TG1 Chemically Competent Cell Operation Instruction
1. Please store TG1 chemically Competent Cells in -80°C for late use.
Take it then insert the tube in the ice immediately for 5 minutes.
2. Add 1µl plasmid DNA (not more than 10ul) to the cell mixture. Carefully flick the tube 4–5 times to mix cells and DNA. Do not vortex.Place the mixture on ice for 25 minutes,Do not mix.
3. Place it in the 42°C water for 45 seconds,then immediately transfer the tube back into ice for 2min. Do not mix.
4. Add 700ul LB or 2YT liquid nutrient medium. then put on the Constant temperature shaker (200 rpm) for 1 hours at 37°C.
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文献和实验Preparing chemically competent cells
Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating
TOP10 chemically competent cells
may not be necessary Place in -80°C freezer indefinitely. Preparing competent cells Inoculate 250 ml of SOB medium with 1 ml vial of seed stock and grow at 20°C to an OD600nm of 0.3 This takes approximately 16 hours.
a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold
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