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- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
6month
- 英文名：
Top10 chemically E.coli Express Competent Cells
- 库存：
货源充足
- 供应商：
LSM Bio
- 规格：
0.1ml*10
Top10 chemically Competent Cells
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文献和实验TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold
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