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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负80摄氏度
- 保质期:
6month
- 英文名:
Top10F chemically E.coli Express Competent Cells
- 库存:
货源充足
- 供应商:
LSM Bio
- 规格:
0.1ml*10
Top10F chemically Competent Cells
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文献和实验a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold
1.5ml缓冲液B,彻底混匀; 30℃水浴孵育1h; 室温下2000g离心10min,去除上清液,菌体沉淀重悬于1.5ml缓冲液C中; 离心样品,去除上清液,轻微操作将样品重悬于0.2ml缓冲液C中; 将所有转化液铺于选择性平板,于30℃孵育3~4天后,鉴定; 3、毕氏酵母电转化法 (1)E.coli TOP10F’感受态细胞的制备 取10ul TOP10F’菌液,接种于200ml LB液体培养基中活化培养,37℃,200 rpm,16~18小时。取100ul菌液接种于200ml液体LB
,etc.).If a cooling shaker is not available- grow the cells at room temperature.More discussions on making competent cell as well as references can be found in TIBS articles "Preparing μltra-competent E.coli" and "Better competent cells"It is also possible
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