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- LSM Bio
- CHC00071
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
6month
- 英文名：
EHA106 chemically Agrobacterium Express Competent Cells
- 库存：
货源充足
- 供应商：
LSM Bio
- 规格：
0.1ml*10
EHA106 chemically Agrobacterium Express Competent Cells
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文献和实验a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold
Yeast Transformation Using Frozen Competent Cells and Single-stranded DNA as a Carrier
Preparing competent cells: 1. Inoculate 100 ml yeast synthetic complete medium + 3% glycerol medium from overnight culture. Grow culture to 0.5-1.0 X 107 cells/ml. (2-3X higher efficiency if diluted back to 2 X 106 cells/ml, then grown
Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
cells when they have reached a density of 1-3 x 106 cells/ml (every 3 to 4 days). Trypan Blue exclusion is used to determine viability and density. . V. PROCEDURES A. Thawing and Establishing Cells 1. Prepare 1L Pro293S media (5 mls
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