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- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
6month
- 英文名：
EHA101 Electro Agrobacterium Express Competent Cells
- 库存：
货源充足
- 供应商：
LSM Bio
- 规格：
0.5ml*10
EHA101 Electro Competent Cells
Packing
EHA101 Electro Competent Cells 50μl/tube
pK7WGF2锛坈ontrol vector锛?0ng/μl ) 10μl
Storage -80鈩冿紙12month锛?/p>
EHA101 Electro Competent Cells Genotype
C58 (rifR) Ti pEHA101 (pTiBo542 D T-DNA) (kanR) Nopaline
Plasmid transformation efficiency
pK7WGF2 (size:11876 bp) >105 cfu/μgDNA锛?/p>
pK7WGF2-ZH锛坰ize:40 kd锛?5×103 cfu/μg DNA
Caution
1.STORAGE AND HANDLING: Competent cells should be stored at–80°C. Storage at –20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above –80°C, even if they do not thaw.
2. This product is FOR RESEARCH USE ONLY!
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文献和实验a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold
the cells appear to make cells more competent.Some cell strains may work better than others (DH5alpha works well in my hand).Note also that some cells (e.g.HB101)has greater recombination activity than others.This method doesn't appear to work with BL
! pH 6.7 - 7.0 Note: Competent Cell s are fragile (cell wall is thought to be weakened),therefore treat the cells gently when preparing these cells.Do not vortex or pippette up and down to resuspend the cells.Do not spin
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