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- 技术资料
- 生长状态：
贴壁生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞形态：
球形
- 细胞类型：
胚胎干细胞
- 器官来源：
胚胎
- ATCC Number：
SCRC-1002™
- 运输方式：
冻存运输
- 库存：
大量
- 品系：
C57/BL6
- 年限：
embryo
| Designations: | C57BL/6 | ||
| Depositors: | JC Conover, B Knowles | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus deposited as mouse | ||
| Morphology: | spherical colony |
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| Source: | Organ: embryo Strain: C57/BL6 Cell Type: embryonic stem cell; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | Prior to purchase, for-profit commercial institutions must obtain a license agreement. For instructions on how to proceed, please contact ATCC 's Office of Licensing and Business Development at licensing@ATCC .org or 703 365 2773. | ||
| Applications: | Coat-color chimera production was high using c2J blastocysts while FVB blastocysts produced a low number of chimeras [PubMed: 11730008]. The clonal embryonic stem cell line #693 ES C57BL/6 was derived from a strain C57BL/6J (B6) mouse blastocyst [PubMed: 11730008]. The ES cells were shown to populate the germ line of two host blastocyst donors, FVB/NJ (FVB) and the coisogenic strain C57BL/6-Tyrc-2J (c2J). |
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| Age: | embryo | ||
| Comments: | The clonal embryonic stem cell line #693 ES C57BL/6 was derived from a strain C57BL/6J (B6) mouse blastocyst [PubMed: 11730008]. The ES cells were shown to populate the germ line of two host blastocyst donors, FVB/NJ (FVB) and the coisogenic strain C57BL/6-Tyrc-2J (c2J). Coat-color chimera production was high using c2J blastocysts while FVB blastocysts produced a low number of chimeras [PubMed: 11730008]. | ||
| Propagation: | ATCC complete growth medium: Mouse ES Cell Basal Medium Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Establishing and maintaining your culture: To insure the highest level of viability, be sure to warm media to 37°C before using it on the cells.
Interval: Every one to two days Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:7 is recommended Medium Renewal: Every day |
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| Preservation: | Freeze medium: Complete growth medium supplemented with an additional 10% FBS and 10% DMSO. Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | recommended serum:ATCC SCRR-30-2020 Recommended medium: ATCC SCRR-2011 recommended serum: ATCC SCRR-30-2020 |
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| References: | 16173597: Brook FA, et al. The derivation of highly germline-competent embryonic stem cells containing NOD-derived genome. Diabetes. 52:205-208, 2003. PubMed: 12502514 16173598: Brook FA, Gardner RL. The origin and efficient derivation of embryonic stem cells in the mouse. Proc. Natl. Acad. Sci. USA. 94: 5709-5712, 1997. PubMed: 9159137 |
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网络 七、国际上常用的F 1 动物 1．AKD2F 1 AKR×DBA/2 2．BA2C F 1 C57BL×A2G 3．BC F 1 C57BL×BALB/C
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outcomes of 9-hour time-restricted feeding of a Western high-fat high-sucrose diet in C57BL/6J mice。该研究发现限时饮食能够保护人体避免脂肪肝、败血症及葡萄糖不耐受等健康问题，动物实验表明限时饮食的保护力与性别、年龄有关。图 4：来源 Cell Reports5. Nature Communications：新技术将塑料垃圾变废为宝塑料垃圾严重破坏生态环境。2021 年 8 月 17 日，清华大学段昊泓教授团队
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