- ￥4000
- 欣润生物
- 江苏
- IP4004
- 2025年11月01日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Immortal pig small intestine smooth muscle cells
- 库存：
10
- 供应商：
欣润生物
- 肿瘤类型：
不是
- 细胞类型：
永生化细胞
- ATCC Number：
111
- 品系：
巴马
- 组织来源：
小肠
- 相关疾病：
无
- 物种来源：
巴马猪猪
- 免疫类型：
正常
- 细胞形态：
梭形
- 是否是肿瘤细胞：
否
- 器官来源：
肠
- 运输方式：
常温
- 年限：
新生
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化猪小肠平滑肌细胞简介：
产品描述：猪小肠平滑肌细胞分离自猪小肠组织。小肠位于腹中，上端接幽门与胃相通，下端通过阑门与大肠相连，是食物消化吸收的主要场所。小肠有三种功能即消化、吸收和分泌及运动功能，其中以吸收和分泌功能为主。平滑肌细胞的收缩是负责肠蠕动的动力，促使食物向下运动。
产品货号：IP4004
产品类型: 原代细胞永生化
传代能力: 30代左右
产品形态: 梭形
培养基：永生化猪小肠平滑肌细胞专用完全培养基，产品编号：IP4004-5
支原体：检测呈阴性
产品培养条件：37℃，5%CO2
发货方式：T25瓶子常温发货
货期：1周左右货期
a-SMA抗体免疫荧光染色鉴定
Establishment of conditionally immortalized epithelial cell lines from the intestinal tissue of adult normal and transgenic mice
It has proved to be impossible to culture epithelial cells from the gastrointestinal tract of adult animals. Researchers have had to use either cell lines derived from newborn rat small intestine or colon carcinoma cell lines that have retained some of the properties of the gastrointestinal mucosa. We have described a method for establishing conditionally immortalized cell lines from the stomach, small intestine, colon, pancreas, and liver from tissue obtained from a transgenic mouse strain carrying a temperature-sensitive mutant of the SV40 large T gene (the "Immortomouse"). This immortalizing gene has proved to be useful for establishing cell lines from a number of transgenic mice following crossbreeding of the Immortomouse with the transgenic mouse of interest. These cell lines are being used in numerous studies. In this review we describe the methods for developing such lines and list the range of cell lines that have been developed from colon, small intestine, stomach, liver, and pancreas of a number of transgenic mice.
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文献和实验Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
技术资料