H9c2 cells, which are rat cardiomyoblast cell lines, were obtained from the Newgainbio company. The cells were grown at 37 °C, and 5% CO2 in the atmosphere using Dulbecco’s modification of Eagle’s medium Dulbecco (DMEM) (4.5 g/L, D-Glucose, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum, penicillin (100 units/ml), and streptomycin (100 ugs/ml) (Gibco, Grand Island, NY, USA).